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Times New Roman K @t @3(TextField2There is a single origin along the circular chromosome of bacteria and viruses where DNA synthesis is initiated. This origin called OriC, the origin of replication, consists of 245 base pairs characterized by the presense of repeating sequences of 9 and 13 bases (called 9mers and 13 mers)^  fRhv 2QГ%8Q4T_O.S.O8SbfoVA> 5jWM,l(~ $ਲ0On ۃm ztHX'|  Xw!rorr fRhv 2QГ%8Q4T_O.S.O8SbfoVA> 5jWM,l(~ $ਲ0On ۃm ztHX'| -i|G9] \p}/hv 2QГ%8Q4T_O.S.O8Sbf2Jtċ_5hR'|A d * 'g)'rW)13h ZSu'&   _s=~fRhv 2QГ%8Q4T_O.S.O8SbfoVA> 5jWM,l(~ $ਲ0On ۃm ztHX'| 5vа["Dd`V?97y]EʎfRhv 2QГ%8Q4T_O.S.O8SbfoVA> 5jWM,l(~ $ਲ0On ۃm ztHX'| 5vа["DdbV}ʎ9ɽCͲ@\p}/hv 2QГ%8Q4T_O.S.O8Sbf2Jtċ_5hR'|A d * 'g)'rW)13h5O\bu'VtE "m7*@& 4cqȸ9ʒ @~@@@@@@@@@@@@@@@@@@@^? K@t @3(TextField2There is a single origin along the circular chromosome of bacteria and viruses where DNA synthesis is initiated. This origin called OriC, the origin of replication, consists of 245 base pairs characterized by the presense of repeating sequences of 9 and 13 bases (called 9mers and 13 mers)^@]^_c?U8ipjCC D-gfJY4y~VkO\RгEҍfE9nȃ8`vP}jLw -C|'qFjӵ3vYRcy<li@@ i@p@ i@@ i@\@ i@@ ipJz "@) Ep"{#EXm^aYe6Xo0,kh O0aYgEk,e]e?  Courier New  3(TextField4Unwinding proteins (called helicase) such as DnaA, DnaB and DnaC, denature the helix at the origin of synthesis, creating the replication fork. Other proteins (SSBPs) stabilize the newly created single strands of denatured DNA.^C?@@@@@@@@@@@@@@@@@@@@@@@@@]TA4b[54b 52aQC2b@C4bd52b"@Cw:$L$:T 3/-0kM(ԧgsDQ=(UvV)2Qs^Km'EQ%,@[@/ f݀k3xi@@ i@p@ i@@ i@\@ i@@ Fhy p"@) E.pS9p 2ah vEl5]-@@@@@@@@@@@@@@@@@@@@@]i@yDi@ يxi@ Ņi@ Gi@ i@@ i@p i@p i@p i@p i@p i@p@ i@ i@ i@ i@ i@ i@@ i@\ i@\ i@\ i@\ i@\ i@\@ i@؉ i@؉ i@؉ i@؉ i@؉ i@@      @@@@@@ţ5 #ţ9bţ5ya ţ5x3 ţ5L@\*5̳\*9#bπ\*5 \*5I \*9#f@({9#({98#]({9É ({9&@ ({9+c@ >9* >9D >9-C@ >9A# >99ـ@90S@9H94 9J 9>@@@@@@ ůse ůwAsB%`@ ~Pspf ~ů(rFH@ ka~1t k^+ t5`@ vr rG@ @!  I`@@@@@@ i@ i@ i@ i@ i@ i@@   i@p i@p i@p i@p i@pUbo2aQC2b@C2b"@CeR5 )OQg#DZ݉X%xI 8ȑ$hldMq6Xݪԛ#$Pڕ41zdZ^TkLW2:#jS糹IJPH٥w`@!  i@p@ i@ i@ i@ i@ i@ i@@ i@( i@( i@( i@( i@( i@(@ i@ i@ i@ i@ i@ i@@  @@@@@@@@@@@@@@BM-jK7N RV,P,@%IjT PTeG%|bbSi@@ i@p@ i@@ i@\@ i@@ jw{ry !"@IT: P!֓U6XnXYYe!#'kY. 5m^a#9 l,, ]̿& _tºa9&   ctºa=18@@@@@@@@@@@@@@@@@@@?@]_cgTf!0AY /gl'Ma{lx@Sp%XE^PVڠ;kLK̎@mh4Lq vӥ`fO4o%*i@@ gn%*i@p@ i@@ i@\@ i@@^ 4o%*N h-y "@0}P`kL|À|hY|u`}P` k] I a!> 3(TextField5As the replication fork moves away from the origin, increased coiling of the helix occurs ahead of the fork. The tension thus created is diminished by the enzymatic action of the topoisomerase enzyme DNA gyrase. This topoisomerase cuts DNA strands, allowing uncoiling to occur, and then reseals the DNA strands.^! @@@@@@@@@@@@@@@@@@@@Cz h C  σ ρ, <$l`@Xp+ 3L- R"xLD7.xLD7.)ffffff-3^/L@""oo鮋Pi@@]p #2gnn鮊Pi@p@ i@@ i@\@?$#xLD7.fff.}.b3# i@@$u"Kws0`(3%ȝ;ݦnS7Vow)@\;MŦqiZn-7@\w;@\;@\;@\;@\W;@\;@\7;@Zo6.âZt24>C}q?naQ$obVFD-}oba;wochVr([;MŦqiZn-7MŦ+;@XǛIx~0;@ZtjӺN ;@ZotkӺN MŦ0 MŠ-hwV@Z`27Z-7i/eZn-7iFAZ'gqh Q7V~ ҅eӴe:`-@FwX/u$[5pX;pX~s1n`XdKpZ-r27\ MŦ0(.qh Vuiȝ˺Znr=-7i@eZn-7i`Fw* FwW FwZʑGMŦqiZn-7MŦP]u$t-7MŦqiZn-7MŦqiZn-6tξ*fUkn2>+9.6ՠ,*ZΠՠ,ˆޅ<sz n-S-7@ z % uv >7V#uh 7V#uh 9J7Vg#uh 37VwVwVowVʯwV/wVwVowVwV/wV^Z{uh dՠ-wV2Zڍ{uh k@-7wVv^Z3uiݦui &\h#; )T%@|ۛzWfsQ*.qiLJv `\]զv aŠ-Fw`wVM`.H]զZ.qh BuiݦZnuQ27wV@]eP#;@]{#;@YZng$/v 3Q7V@XP LܦnS7)X @Y L09Am N.E=BeCƣ`,DZn-7iqh (wj z $N- qh &gqh$i@ "oo鮋P@ i@C t C  σ ρ8 `<%l  Xs+4L.` i@hd kkzQ.k,F@y@ i@4C n C1 # σ ρ[ !<'؈lX"8X|`.6؈L0 i@̈B4bbLRxL&cB@!@ i@̈C䀇 ZҀ Cl ^ σ ρ %H<+l%X 1Ȉ:L4H i@42VyVyT Sqf*@@ i@hD7 % C  τA ρ逈 *x<0l8+X6?L9x i@k,?E?EW@ 3(TextField5As the replication fork moves away from the origin, increased coiling of the helix occurs ahead of the fork. The tension thus created is diminished by the enzymatic action of the topoisomerase enzyme DNA gyrase. This topoisomerase cuts DNA strands, allowing uncoiling to occur, and then reseals the DNA strands.]% &&   A^&j =6U@&' b'j =PV`@@]^b(Mx7(% +]f(pXӻc49)M;dH0AY ).?8aNm4ә?)w:|_ppG?:(pXӻj8(pXӻhSF(pXӻb  (pXӻj!(pXӻj9V"(pXӻb*<#(pXӻa_4p$(pXӻbԿH%(pXӻic&(pXӻix'(pXӻjzL\((pXӻjv)(pXӻj(*)#腄8+)D; n*x `x `7ffffff-h!}x(t.q9jte:bh!v';,)԰:: # ]* +1 3(TextField6Initiation of DNA synthesis occurs concurrently on both template strands and involves an RNA primer synthesized under the direction of a unique RNA polymerase called primase. The resultant RNAs are complementary to their DNA templates.c+ C`@]p@]@]@]?2,x `fff Υ1耎e:^=1],@]@@@@@@S-hZy "@@Ո}hYuP`kPxYD8 Y|pYu`}P` k]-I@@@@@@@@@@@@@@@@@@@@@@@@]@@@@@ .X  "@9Λ k ].@@@@@@@@@@@@@@@@@@@@@@@@@*+]X)w:|_ppG?:Y)#腄8Z)D;@XJK'̴|Yeg+mμؑZf3ڦW+@Xjŝe1Nr&Y@@mK]`ZP9+\@Xu:C^;r(Y N˪xZxy{@XJ!%}vq5Y+\`Og+XZ̘LFѹ1@X<Z6"GYË`/*Y`Zj52 ȝF@@@@@N(j j JqHf@pXӻj2NfpXӻjmUnpXӻiZ|pXӻcq6 pXӻj֑!pXӻj"pXӻizPr#pXӻb)H$pXӻiϑ%pXӻiӼ&pXӻj-'pXӻjߍ(pXӻjP)pXӻjX=!$)c"Yû|`/)YZ3uͰ@pXӻjpXӻjҖpXӻiղpXӻj pXӻk\ !pXӻkS‘"pXӻiߑ#pXӻb> $pXӻj4>%pXӻj.&pXӻjT'pXӻkDȑ(pXӻk\)pXӻjLI(T8pNGʼn;AqpO(Gʼn;AqS(ƺO]'v(sz5X=!$)~Yû|`/) YZ3u͘`@pXӻjґpXӻk7ڑpXӻj%pXӻj* pXӻkWB!pXӻki"pXӻjDޑ#pXӻiP$pXӻjt%pXӻjV(&pXӻj'pXӻk(pXӻk)pXӻkXRX=!$)ǚYû|`/(JYؑZ3u@pXӻkbpXӻkpXӻjXpXӻjQ` pXӻrF!pXӻk."pXӻj#pXӻjD$pXӻjT%pXӻj^&pXӻk\P'pXӻrM(pXӻr)pXӻkJ(Q`X=!$)Ƕ#vYû|`/'ZZ3ugv@ !"#$%&'()T(ƺO]'v(sgX=!$)ѣYû|`/&Z0Z3uN@K(N`P(Gʼn;Aq X=!$)$fYû|`/&Z`Z3u6@U(ƺO]'v(sU&X=!$)ޑYû|`/%FZZ3u@L(Kȱ`Q(Gʼn;AroX=!$)$%VYû|`/$ZZ3u@V(ƺO]'v(sBf'X=!$)@%ΑYû|`/#ZZ3u0@JQh`KN`M(I 0R(Gʼn;ArɿX=!$)[FYû|`/#[Z3u@W(ƺO]'v(s2%X<Z6"w&YË`/"F[@Zj52 ̻F@@@@@@XYZ/P C]/W0P C^0aW_0`/B]a0yb/c@@@@@@@@@@@@?&1 Bd1t`= xJ&2   h2t`=;K@]^_`abc3Mx7(% +]f3pXӻc494M;dH0 6p œ i3v9*l?4w:|_ppG?:3pXӻj83pXӻhSF3pXӻb  3pXӻj!3pXӻj9V"3pXӻb*<#3pXӻa_4p$3pXӻbԿH%3pXӻic&3pXӻix'3pXӻjzL\(3pXӻjv)3pXӻj(*4#腄8+4D; n5x `x `7ffffff-h!}x(t.q9jte:bh!v';,)԰:: # ]5 6h 3(TextField8DNA polymerase III polymerizes complementary DNA strands by simultaneously elongating the existing primer in the 5' to 3' direction^6 #2`@dh]p@]@]@]],@]@@@@@@G7h "@LYkYLY(8 Y|pYu`}P`]7q@@@@@@@@@@@@@@@@@@@@@@@@]@@@@@8`P0 "@ y]8@@@@@@@@@@@@@@@@@@@@@@@@@*+]X4w:|_ppG?:Y4#腄8Z4D;@XƲcY1ł >sNDrYeg+mbؒZŚ|.ghse@Xg3HnW- PȑY@@mK]`Z2f@XM&²MY N,xZe՗q~\>`@Xe2Zr@eY+\`Og2zXZs1~i\n`@Xƅ'Bƪr.eYË`/1Y`Zz4l_>`"@@@@@*3 8jǙ–l03PG(#~Ase@pXӻjxpXӻjȗpXӻi֎pXӻjБ pXӻkR!pXӻjT"pXӻiՒ#pXӻipd$pXӻj*%pXӻj$Α&pXӻjU'pXӻk:Ϥ(pXӻkR)pXӻj(93żE&ȕXƆ7Cƪʪr&eYû|`/1YXZz_:Pb$@pXӻkБpXӻkRؑpXӻj?XpXӻjR( pXӻkۙ@!pXӻk"pXӻj_ܑ#pXӻi$pXӻjUr%pXӻj&&pXӻk'pXӻkQ(pXӻk)pXӻkr+3xYû|`//YXZz_:Pb&Ÿ@pXӻkpXӻkpXӻjZƑpXӻk T pXӻr$!pXӻk֑"pXӻj#pXӻjg$pXӻk"WR%pXӻk&pXӻk'pXӻr$(pXӻr%)pXӻk`,3ƺ]Zʼn;;cr f23a"m˿0<3@XƆ7Cƪʪr aYû|`//>YXZz_:Pb(@ !"#$%&'()=3@XƆ7CƪʪrYû|`/.YXZz_:Pb)|@-3ƮWo~@kx33a"mo0>3چ@XƆ7CƪʪkڤYû|`/-YXZz_:Pb*؟@?3*@XƆ7Cƪʪk4Yû|`/-^YXZz_:Pb,6@.37`Ō9kڤ43a"m0@3ʆ@XƆ7Cƪʪk‘Yû|`/,YXZz_:Pb-@A3j@XƆ7CƪʪkRYû|`/,YXZz_:Pb.@/37`Ō9k B3 @XƆ7Cƪʪk\Yû|`/+~YXZz_:Pb0L@C3'!U(Xƅ'BƪknYË`/*Y`Zz4l_>`1@ZBAň;s.@ZA̠ňU;s8ǀ@D3Wƅ@s*ZA̠ňU;sBɕ@E3Wƅ@s8iZA̠ňU;sLc@F3Wƅ@sGiGZA̠ňU;sV1@G3Wƅ@sZ(ZA̠ňU;s`@H3Wƅ@sn(ZA̠ňU;sj̀@ZA̠ňU;stț@ZBAň;s~i@@@@@?&9 `9qȸ= C&:   d:qȸ=;C@XYZ^O;xoDg(%8Ge@^-0>5@ٵ8U@Xʴ:P*h5qX];4<h@ Fh5!-ƌ7nd!$b^<4=h@ Fh5!FBƌ7nd!$b_=@`d@@@@@@@@@@@@@@@@@@@@@@@]^_ >U ,(TextField9As the replication fork moves away from the origin, synthesis is continuous on the leading strand, but is discontinuous on the lagging strand, producing short polynucleotides called Okazaki fragments.a>@@@@@@ ?\rq%qzc:\#> @?@4?@Ōf4?@Ōf4@f@Ōf@f@@f@@?@]@,@@@@@@@a pAoAS (TextField10Discontinuous synthesis of DNA requires the removal of the RNA primer aA8@  *+,-./NOPQR[\] X X` =*0 0p (RЍ $L Vl@T*k8w!uЀ 1}0PF_' t701`-HA2`-H#A3`-HcA4`-HA53`-HC>63`-H>73`-Hތ83`-H 9 ^c\: c\; c\< Jc\= c\> c\? c\@ c\A Rc\Bzr8$}3`CWU2`!DWU2`#8EWU2`%xFWU2`'xGWU2`*d8H c\I`@J`?K`:EL`4M`/-@SʺN,iTʺN)^UʺN'RShVʺN$H(WʺN">ȔX)ɻhn2P/АY4 >Bx-_Q (%kv_@ZB[ @8W0`-HA1`-H#A2`-HcA3`-HA4`-HC>5`-H>6`-Hތ7`-H SʺN(jYTʺN&NhUʺN#C(VʺN!9@670`-HcA1`-HA2`-HC>3`-H>4`-Hތ5`-H SʺN$ETʺN"R:hU6ͯ+4V6ͯ3@45V0`-HC>1`-H>2`-Hތ3`-H S6ͯɬ4T6ͯɫ 3U6ͯɭ6P@23U0`-H1`-HS`PgT`-@@@@a? pCoAS (TextField10Discontinuous synthesis of DNA requires the removal of the RNA primer \C8&D   <]D@;@\]@Xɻcn?s @Xɻcn?ͪ@Xɻcn?qՠ@Xɻhn2-p@@@@@Xɻcn?@N(ʶ䭹2+l(Xɻcn?@Xɻcn?@O(ʶ䭹2)`Xɻcn?z@Xɻcn?h@P(ʶ䭹2'RUXɻcn?V@Xɻcn?D@Q(ʶ䭹2%2KxXɻcn?2@Xɻcn? @R(ʶ䭹2#:BxXɻhn2!C@@@@@ Xɻcn? i@@@ Xi@@ Xi@@@ Xɻhn2!Ci@@X@Y 0`@Y &a0@Y  B@Y @@@@ Yi@@Y @3D&2ޠY @Y @Y @3`\>gY @Y @3`YPgY @Y @3`VgY @Y @@@@@@@@@@@)EppȒ(%T$;-L*I 0\E;Fh~ "@?@ABCDEFGHIJPQR[\rDH z,"N`0ʑ00[0`@l $qk"1`@l $q+"23`@l $q="֓33`@l $q"֕K3ζV܌|pzL3ζV܌|pz`M3ζV܌|pz,`N3ζV܌|pzw`O3ζV܌|pzW`S0~nT0bU(0nV(02bY BB3\tqː#Z)ʅǡqvq#Γi@@@@@@@V530U02bYAs3^Ot9ʽ#Zʈsvב%@YAs3^Ot9#Zʈsvב%>@U630QYAs3^Ot9#Zʈsvב%@YAs3^Ot9G#Zʈsvב%@T730:QS0bYAs3^Ot9u#Zʈsvב%$@YAs3^Ot9ƣ#Zʈsvב%$@S830QYAs3^Ot9#Zʈsvב%k$@Y BB3\tq#Zʅǡqvq#G$@@@@@@ YAs3^Ot9i@ @ Yi@@ Yi@ @ Y BB3\tq#i@@Y@Zʈsvב%FU @Zʈsvב%Fw@Zʈsvב%ţ#@Zʅǡqvq#ţ@@@@E30Qmi@@ E0ґn HP@ E@ 0@ E <@ E0Qm9@@@@ E0ґn6@ E@+@@ E$ p@@ E0Qm@@@@@@@@@@@@ Zʈsvב%Ťi@ @ Zi@@ Zi@ @ Zʅǡqvq#ţi@@Z@@@@@@@@@@@`U5Frq…}y` Z Y  9' ڹIZ-fJ@ʱS7ЀTU$\}@! 8jǙ–lx3 @?3'!U(@3Wƅ@s*A3Wƅ@s8iB3Wƅ@sGiGC3Wƅ@sZ(D3Wƅ@sn(ET80F3Qh G3N H3KȰ I3I 0J3V0KGʼn;AqpLGʼn;AqMGʼn;Aq NGʼn;AroOGʼn;ArɿP3ƺO]'v(sz5Q3ƺO]'v(sgR3ƺO]'v(sU&S3ƺO]'v(sBf'T3ƺO]'v(s2%Kr Lp(%ʾu}UKVНW/WX07Y0Ӂ΀Z/@@@@@@@@@@@@@@@@@@@@@@@@@@?&L   4[L 3Mg? @(TextField14Play again_M{B@@[_@@@@@@@@@@@@@@      0123456789:;<=>?@ABCDEFGHIJKLMNOPQRSTUVWXYZ@